Yes.
Glasses or contact lenses are not a handicap when using eye trackers. However, you should be aware of a few points:
- The lighting conditions in your environment should not produce any reflections on your glasses that would make your pupil unrecognizable to the eye tracker
- Glasses with a small frame could obscure the pupil from the eye tracker during head movements
- Bifocal glasses may confuse the eye tracker in a few cases
- The manufacturer for the Tobii 4C eye tracker offers additional points in the following link: https://help.tobii.com/hc/en-us/articles/210249865-Glasses-lenses-and-eye-surgery